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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00011183-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011183-M01, RRID:AB_2235052
- Product name
- Mouse Monoclonal MAP4K5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAP4K5 - mitogen-activated protein kinase kinase kinase kinase 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Western Blot analysis of MAP4K5 expression in 293 ( Cat # L026V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.Lane 1: MAP4K5 transfected lysate(95.04 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MAP4K5 Antibody (3H3) [H00011183-M01] - Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.
