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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-22016 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAP4K5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HepG2, Molt-4, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAP4K5 Polyclonal Antibody (Product # PA5-22016). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. MAP4K5 antibody. MAP4K5 Polyclonal Antibody (Product # PA5-22016) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KHS/MAP4K5 in paraformaldehyde-fixed A431 cells using a KHS/MAP4K5 polyclonal antibody (Product # PA5-22016) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS antibody (Product # PA5-22016) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of KHS was performed in 293T whole cell extracts using 5 µg of MAP4K5 Polyclonal Antibody (Product # PA5-22016). Samples were transferred to a membrane and probed with MAP4K5 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
