PA5-67167

antibody from Invitrogen Antibodies

Targeting: E4F1 E4F

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-67167

Provider

Invitrogen Antibodies

Product name

E4F1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RHLKSLTPCTE KIRFSVSKDV VVSKEDARAG SGAGAAGLGT ATSSVTGEPI ETSPVIHLVT DAKGTVIHEV HVQM

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of E4F1 in human cell line RT-4 and human cell line U-251 MG. Samples were probed using an E4F1 Polyclonal Antibody (Product # PA5-67167).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of E4F1 in human cell line MCF7 shows localization to nucleoplasm. Samples were probed using an E4F1 Polyclonal Antibody (Product # PA5-67167).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RNA immunoprecipitation (RIP) western of E4F1 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal E4F1 antibody (Product # PA5-67167) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti- rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RNA immunoprecipitation (RIP) western of E4F1 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal E4F1 antibody (Product # PA5-67167) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti- rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.