Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- R20226 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H4K16ac Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant H4K16ac antibody is suitable for use in Western blot/ELISA applications with all species samples.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM204
- Vial size
- 25 ug, 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H4K16ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot test of acid extracts from (1) HeLa cells treated with sodium butyrate and (2) HeLa cells untreated using recombinant H4K16ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- This recombinant H4K16ac antibody specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl) or other acetylated Lysines.