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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [2]
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- Product number
- TA347211 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal H4K8ac Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal H4K8ac Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HIST4H4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against H4K8ac diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human H4K8ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays using human U2OS cells (sheared chromatin from 100,000 cells treated with the deacetylase inhibitor ATRA). A titration of 0.5, 1.5 and 5ul antibody per ChIP was performed after incubation with 5 nM blocking peptide for 1 hr at RT. IgG (5 ug/IP) as negative control. qPCR primers were for ALDOA promoter (fructose-bisphosphate aldolase A) and for the coding region of MYOD (inactive at normal conditions). Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR).
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- A Dot Blot analysis was performed to test the cross reactivity of the antibody against H4K8ac with peptides containing other modifications of histone H4 and H3 and an unmodified histone H4 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Image shows a high specificity of the antibody for the modification of interest.
- Validation comment
- DB
