Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-61553 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal Histone H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [p Ser1] Antibody (194) [NBP2-61553] - (1) acid extracts of HeLa cells; (2) recombinant Histone H4, at 0.5 ug/mL, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [p Ser1] Antibody (RM194) [NBP2-61553] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61553, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Histone H4 [p Ser1] Antibody (194) [NBP2-61553] - Specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Multiplex Immunoassay: Histone H4 [p Ser1] Antibody (RM194) [NBP2-61553] - NBP2-61553 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
