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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Other assay [1]
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- Product number
- NBP2-61556 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal Histone H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 Antibody (195) [NBP2-61556] - (1) acid extracts of HeLa cells; (2) recombinant Histone H4 at 0.5 ug/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. using NBP2-61556, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Histone H4 Antibody (195) [NBP2-61556] - Specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Multiplex Immunoassay: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - NBP2-61556 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a), symmetric dimethylated Arg 3 (R3me2s), or citrullinated Arg 3 (R3Cit) in Histone H4.
