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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab108080 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Histone H4 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Histone H4 Antibody, Recombinant(R05-8H9), could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 60-76- Antibody clone number
- R05-8H9
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Histone H4 Antibody (Ab108080) - Western Blot All lanes: Recombinant Histone H4 Antibody (Ab108080) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 14 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Histone H4 Antibody (Ab108080) - IF/ICC Immunocytochemistry analysis of Histone H4 (green) in HeLa using Recombinant Histone H4 Antibody (Ab108080), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Histone H4 Antibody (Ab108080) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant Histone H4 Antibody (Ab108080). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
