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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- Ab156574 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Histone H4 Mouse mAb
- Antibody type
- Monoclonal
- Description
- ApplicationWB: 1/1,000-1/200,000 ; Predicted molecular weight: 11.4 kDaICC/IF: 1.0 _g/mL
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CD01/2H7
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at +4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Histone H4 Mouse mAb (Ab156574) - Western Blot All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 11.4 kDa Observed band size: 13.6 kDa Exposure time: 2.4 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Histone H4 Mouse mAb (Ab156574) - Western Blot All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution Samples: Lysate at 20 µg Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 11.4 kDa Observed band size: 13.5 kDa Exposure time: 60.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Histone H4 Mouse mAb (Ab156574) - ICC/IF IF analysis of Histone H4 (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Histone H4 Mouse mAb (Ab156574) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
