PAB29488

antibody from Abnova Corporation

Targeting: CD207 CLEC4K, Langerin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29488

Provider

Abnova Corporation

Product name

CD207 polyclonal antibody

Antibody type

Polyclonal

Description

CD207 polyclonal antibody raised against recombinant human CD207.

Antigen sequence

VKTNVQLLKGRVDNISTLDSEIKKNSDGMEAAGVQ
IQMVNESLGYVRSQFLKLKTSVEKANAEIQILTRS
WEEVSTLNAQIPELKSDLEKASALNTKIRALQGSL
ENMSKLLKRQNDILQVVSQGWKYFKGNFYYFSLIP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot (Cell lysate) analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CD207 polyclonal antibody (Cat# PAB29488) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS cells with CD207 polyclonal antibody (Cat# PAB29488) under 1-4 ug/mL working concentration shows positivity in cytoplasm. Antibody staining is shown in green.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with CD207 polyclonal antibody (Cat# PAB29488) shows distinct cytoplasmic positivity in a subset of cells that resembled Langerhans cells at 1:20 - 1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)