352202

antibody from BioLegend

Targeting: CD207 CLEC4K, Langerin

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

352202

Provider

BioLegend

Proper citation

BioLegend Cat#352202, RRID:AB_10898115

Product name

Purified anti-human CD207 (Langerin)

Antibody type

Monoclonal

Antigen

Primary human Langerhans cells

Description

CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Monocyte-derived Langerhans cells were stimulated with recombinant human GM-CSF, TGF-β and IL-4 for 3 days, followed by an additional 3-day culture with rhGM-CSF and TGF-β. They were then stained with CD1a APC and CD207 (clone 10E2) PE (top) or mouse IgG1, κ PE isotype control (bottom).

Conjugate

Unconjugated