Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-63019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFAF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MEEILKNEKH REEIKIKSQD FYEKEKLLSK ETSEELLPPP VQTQIKGHAS APYFGKEEPS VAPSSTGKTF QPGSWMPRDG KSHNQ Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFAF2 using NDUFAF2 Polyclonal Antibody (Product # PA5-63019) (A) shows similar pattern to an independent NDUFAF2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NDUFAF2 Polyclonal Antibody (Product # PA5-63019) and a 20 kDa band corresponding to NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 was observed. For Fig a, Whole cell extracts (30 µg lysate) of Raji (Lane 1), PC-3 (Lane 2), HEK-293 (Lane 3), SK-BR-3 (Lane 4), HeLa (Lane 5), T98G (Lane 6), MCF7 (Lane 7) and for Fig b, Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NDUFAF2 in human cell line HEK 293 shows positivity in mitochondria. Samples were probed using a NDUFAF2 Polyclonal Antibody (Product # PA5-63019).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NDUFAF2 Polyclonal Antibody (Product # PA5-63019) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NDUFAF2 in human kidney tissue shows strong granular cytoplasmic positivity in cells in tubules. Samples were probed using a NDUFAF2 Polyclonal Antibody (Product # PA5-63019).