Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7804 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RPAIN Antibody / RPA-interacting protein
- Antibody type
- Polyclonal
- Description
- This highly specific RPAIN antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the RPAIN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A375, 2) human SH-SY5Y, 3) human Jurkat, 4) human MOLT4, 5) mouse ovary and 6) mouse testis tissue lysate with RPAIN antibody. Predicted molecular weight: 16-26 kDa (multiple isoforms), but can be observed at 30-40 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human RT4 cells with RPAIN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPAIN antibody.