PA5-31241

antibody from Invitrogen Antibodies

Targeting: WBP11 BUG13, CFAP90, FAP90, NPWBP, PPP1R165, SIPP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31241

Provider

Invitrogen Antibodies

Product name

WBP11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji. Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (85%), Rhesus Monkey (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WBP11 using 30 µg of A) Jurkat (B) Raji (C) K562 (D) THP-1 and E) HL-60 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WBP11 polyclonal antibody (Product # PA5-31241) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WBP11 using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WBP11 polyclonal antibody (Product # PA5-31241) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WBP11 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WBP11 Polyclonal Antibody (Product # PA5-31241) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of WBP11 was performed in paraffin-embedded mouse brain tissue using WBP11 Polyclonal Antibody (Product # PA5-31241) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Western BlotP11 (Product # PA5-31241) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western BlotP11 Polyclonal Antibody detects Western BlotP11 protein at nucleus in rat adrenal by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal. Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western BlotP11 Polyclonal Antibody detects Western BlotP11 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western BlotP11 Polyclonal Antibody immunoprecipitates Western BlotP11 protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of Western BlotP11 protein by 4 µg Western BlotP11 Polyclonal Antibody (Product # PA5-31241). 7.5 % SDS-PAGE. The immunoprecipitated Western BlotP11 protein was detected by Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:1,000.