PA1-46215

antibody from Invitrogen Antibodies

Targeting: PLIN5 LSDA5, LSDP5, MLDP, OXPAT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-46215

Provider

Invitrogen Antibodies

Product name

OXPAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The target sequence has 94% sequence homology with sheep and porcine and 84% sequence homology with rat. Suggested positive control: human and mouse heart lysate.

Reactivity

Human, Mouse, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet.

Espinosa A, Ross A, Dovale-Rosabal G, Pino-de la Fuente F, Uribe-Oporto E, Sacristán C, Ruiz P, Valenzuela R, Romero N, Aubourg SP, Rodríguez A
Molecules (Basel, Switzerland) 2020 Jul 20;25(14)

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Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma.

Asimakopoulou A, Vucur M, Luedde T, Schneiders S, Kalampoka S, Weiss TS, Weiskirchen R
Cancers 2019 Mar 19;11(3)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OXPAT in 0.5 mg/mL Human Heart (left) and Mouse Heart (right) lysate. Samples were incubated in OXPAT polyclonal antibody (Product # PA1-46215). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OXPAT in human (lane 1) and mouse (lane 2) heart lysate. Samples were incubated in OXPAT polyclonal antibody (Product # PA1-46215).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OXPAT in 0.5 mg/mL Human Heart (left) and Mouse Heart (right) lysate. Samples were incubated in OXPAT polyclonal antibody (Product # PA1-46215). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of OXPAT in paraffin embedded mouse fat. Samples were incubated in OXPAT polyclonal antibody (Product # PA1-46215).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Perilipin levels in freshly isolated hepatocytes from high-fat diet (HFD)-fed mice. Animal groups were fed with HFD and divided into four groups. Oral oil administration: A, sunflower oil; B, DSO; C, CDSO concentrated by urea complexation and D, CPUFA Salmon oil concentrated by urea complexation. Lysates from isolated hepatocytes were used to determine Plin5 ( a ) and Plin2 ( b ) protein levels by Western blot. The top image shows the original gel plus the respective Ponceau red stain, and the bottom image shows the densitometric analysis. Data are presented as mean +- SEM; * p < 0.05, by one-way analysis of variance (ANOVA) followed by Tukey post hoc test.