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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-85682 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 2.24 mg/mL
Submitted references Alzheimer's disease-causing presenilin-1 mutations have deleterious effects on mitochondrial function.
Han J, Park H, Maharana C, Gwon AR, Park J, Baek SH, Bae HG, Cho Y, Kim HK, Sul JH, Lee J, Kim E, Kim J, Cho Y, Park S, Palomera LF, Arumugam TV, Mattson MP, Jo DG
Theranostics 2021;11(18):8855-8873
Theranostics 2021;11(18):8855-8873
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATL1 Polyclonal Antibody (Product # PA5-85682). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATL1 Polyclonal Antibody (Product # PA5-85682) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATL1 Polyclonal Antibody detects ATL1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATL1 protein stained by ATL1 Polyclonal Antibody (Product # PA5-85682) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 ATL2 expression level is elevated in the brains of AD mouse model and AD patients. (A) Representative western blots of ATL1, ATL2, ATL3, and Tau in the hippocampi of seven-month-old 3xTg-AD mice (male, n = 2; female, n = 4) and age-matched wild-type (male, n = 2; female, n = 4). (B) Quantification of ATL1, ATL2, and ATL3 expressions in (A); **** P < 0.0001; Student's t -test (two-tailed). (C) Representative western blots of ATL1, ATL2, ATL3, and Tau in the hippocampi of twelve-month-old 3xTg-AD mice (male, n = 2; female, n = 4) and age-matched wild-type (male, n = 2; female, n = 4). (D) Quantification of ATL1, ATL2, and ATL3 expressions in (C); **** P < 0.0001; Student's t -test (two-tailed). (E) Representative western blots of ATL1, ATL2, ATL3, and BACE1 in the inferior parietal lobule of AD patients (n = 3) and age-matched control subjects (n = 3). (F) Quantification of ATL1, ATL2, and ATL3 expressions in (C); * P < 0.05; Student's t -test (two-tailed). (G) Expression levels of ATL1 , ATL2 , and ATL3 in the frontal white matter of subjects with (red, n = 33) and without (blue, n = 36) AD; * P < 0.05; Student's t -test (two-tailed). The values shown indicate the means +- SEM.
