PA5-85682

antibody from Invitrogen Antibodies

Targeting: ATL1 AD-FSP, FSP1, SPG3, SPG3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85682

Provider

Invitrogen Antibodies

Product name

ATL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Mouse (96%), Rat (98%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.24 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Alzheimer's disease-causing presenilin-1 mutations have deleterious effects on mitochondrial function.

Han J, Park H, Maharana C, Gwon AR, Park J, Baek SH, Bae HG, Cho Y, Kim HK, Sul JH, Lee J, Kim E, Kim J, Cho Y, Park S, Palomera LF, Arumugam TV, Mattson MP, Jo DG
Theranostics 2021;11(18):8855-8873

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ATL1 Polyclonal Antibody (Product # PA5-85682). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATL1 Polyclonal Antibody (Product # PA5-85682) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ATL1 Polyclonal Antibody detects ATL1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATL1 protein stained by ATL1 Polyclonal Antibody (Product # PA5-85682) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 ATL2 expression level is elevated in the brains of AD mouse model and AD patients. (A) Representative western blots of ATL1, ATL2, ATL3, and Tau in the hippocampi of seven-month-old 3xTg-AD mice (male, n = 2; female, n = 4) and age-matched wild-type (male, n = 2; female, n = 4). (B) Quantification of ATL1, ATL2, and ATL3 expressions in (A); **** P < 0.0001; Student's t -test (two-tailed). (C) Representative western blots of ATL1, ATL2, ATL3, and Tau in the hippocampi of twelve-month-old 3xTg-AD mice (male, n = 2; female, n = 4) and age-matched wild-type (male, n = 2; female, n = 4). (D) Quantification of ATL1, ATL2, and ATL3 expressions in (C); **** P < 0.0001; Student's t -test (two-tailed). (E) Representative western blots of ATL1, ATL2, ATL3, and BACE1 in the inferior parietal lobule of AD patients (n = 3) and age-matched control subjects (n = 3). (F) Quantification of ATL1, ATL2, and ATL3 expressions in (C); * P < 0.05; Student's t -test (two-tailed). (G) Expression levels of ATL1 , ATL2 , and ATL3 in the frontal white matter of subjects with (red, n = 33) and without (blue, n = 36) AD; * P < 0.05; Student's t -test (two-tailed). The values shown indicate the means +- SEM.