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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
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- Product number
- LS-C312910 - Provider product page
- Provider
- LSBio
- Product name
- ECI1 / DCI Antibody (aa272-290) LS-C312910
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of DCI / ECI1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Human Placenta Tissue Lysate. Lane 3: A549 Cell Lysate. Lane 4: SMMC Cell Lysate . Lane 5: COLO320 Cell Lysate . Lane 6: HELA Cell Lysate . Lane 7: HT1080 Cell Lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of DCI using anti-DCI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DCI / ECI1 antibody. ICC: HELA Cell.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DCI / ECI1 antibody. ICC: A549 Cell.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DCI / ECI1 antibody. ICC: HELA Cell.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DCI / ECI1 antibody. ICC: A549 Cell.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DCI / ECI1 antibody. IHC(P): Human Breast Cancer Tissue.
