PA5-79184

antibody from Invitrogen Antibodies

Targeting: ECI1 DCI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-79184

Provider

Invitrogen Antibodies

Product name

DCI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCI in Lane 1: rat liver tissue lysate, Lane 2: human placenta tissue lysate, Lane 3: A549 whole cell lysate, Lane 4: SMMC whole cell lysate, Lane 5: COLO320 whole cell lysate, Lane 6: HeLa whole cell lysate, Lane 7: HT1080 whole cell lysate using 40-50 µg per well. Sample was incubated with DCI (Product # PA5-79184) at a dilution of 0.5 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DCI in HeLa cells. Sample was incubated with DCI polyclonal antibody (Product # PA5-79184).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DCI in A549 cells. Sample was incubated with DCI polyclonal antibody (Product # PA5-79184).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DCI using anti-DCI antibody (Product # PA5-79184) . DCI was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DCI antibody (Product # PA5-79184) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DCI using anti-DCI antibody (Product # PA5-79184). DCI was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DCI antibody (Product # PA5-79184) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.