OAAB16652

antibody from Aviva Systems Biology

Targeting: CLCN4 ClC-4, CLC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB16652

Provider

Aviva Systems Biology

Product name

CLC4 antibody - C - terminal region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) (Cat.# OAAB16652). Data courtesy of Emily McMains, Louisiana State University.

Immunocytochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (Cat # OAAB16652). Data courtesy of Emily McMains, Louisiana State University.

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# OAAB16652) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)