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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- PA5-58868 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERP27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ILVDSGMKEN GKVISFFKLK ESQLPALAIY QTLDDEWDTL PTAEVSVEHV QNFCDGFLSG KLLKENRESE GKTPKV Highest antigen sequence identity to the following orthologs: Mouse - 66%, Rat - 57%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERP27 in control (vector only transfected HEK293T lysate) and ERP27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ERP27 Polyclonal Antibody (Product # PA5-58868).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human liver using ERP27 Polyclonal Antibody (Product # PA5-58868).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Samples were probed using an ERP27 Polyclonal Antibody (Product # PA5-58868).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human testis using ERP27 Polyclonal Antibody (Product # PA5-58868).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human pancreas and colon tissues using ERP27 Polyclonal Antibody (Product # PA5-58868). Corresponding ERP27 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human colon, liver, pancreas and testis using ERP27 Polyclonal Antibody (Product # PA5-58868) (A) shows similar protein distribution across tissues to an independent ERP27 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ERP27 in human colon using ERP27 Polyclonal Antibody (Product # PA5-58868) shows low expression as expected.
