Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX118734 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118734, RRID:AB_10624937
- Product name
- HAUS2 antibody [N1C3-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.
Tsai CY, Ngo B, Tapadia A, Hsu PH, Wu G, Lee WH
The Journal of biological chemistry 2011 Aug 26;286(34):30097-106
The Journal of biological chemistry 2011 Aug 26;286(34):30097-106
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GTX118734 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:500.Blue: Hoechst 33343 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.