GTX118734

antibody from GeneTex

Targeting: HAUS2 C15orf25, CEP27, FLJ10460, HsT17025

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX118734

Provider

GeneTex

Proper citation

GeneTex Cat#GTX118734, RRID:AB_10624937

Product name

HAUS2 antibody [N1C3-2]

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.

Tsai CY, Ngo B, Tapadia A, Hsu PH, Wu G, Lee WH
The Journal of biological chemistry 2011 Aug 26;286(34):30097-106

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GTX118734 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:500.Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.