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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
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- Product number
- PA5-31258 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAUS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Pig (82%), Rhesus Monkey (85%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HAUS2 showing staining in the cytoplasm of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a HAUS2 polyclonal antibody (Product # PA5-31258) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HAUS2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HAUS2 Polyclonal Antibody (Product # PA5-31258) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAUS2 Polyclonal Antibody detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: HAUS2 protein stained by HAUS2 Polyclonal Antibody (Product # PA5-31258) diluted at 1:500. Blue: Hoechst 33343 staining.
