Explore
Validate
LearnAF1147
antibody from Novus Biologicals
Targeting: MYDGF
C19orf10, IL-25, IL-27, IL25, IL27, IL27w, R33729_1, SF20
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1147 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL25 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Myeloid-derived growth factor is a resident endoplasmic reticulum protein.
Bortnov V, Annis DS, Fogerty FJ, Barretto KT, Turton KB, Mosher DF
The Journal of biological chemistry 2018 Aug 24;293(34):13166-13175
The Journal of biological chemistry 2018 Aug 24;293(34):13166-13175
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SF20 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20 Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SF20 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
