MA5-29431

antibody from Invitrogen Antibodies

Targeting: MYDGF C19orf10, IL-25, IL-27, IL25, IL27, IL27w, R33729_1, SF20

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29431

Provider

Invitrogen Antibodies

Product name

C19orf10 Monoclonal Antibody (8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

8

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human ARD1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with C19orf10 Monoclonal Antibody (8) (Product # MA5-29431, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human ARD1A expression on HeLa cells. The cells were treated according to manufacturer’s manual, stained with C19orf10 Monoclonal Antibody (8) (Product # MA5-29431), then a PE-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.