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LearnRQ8382
antibody from NSJ Bioreagents
Targeting: MYDGF
C19orf10, IL-25, IL-27, IL25, IL27, IL27w, R33729_1, SF20
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- RQ8382 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MYDGF Antibody / Myeloid-derived growth factor
- Antibody type
- Polyclonal
- Description
- This highly specific MYDGF antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MYDGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat NRK, 3) mouse ovary, 4) mouse stomach and 5) mouse liver tissue lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Jurkat, 3) HepG2, 4) PC-3, 5) U-251, 6) ThP-1, 7) A431 and 8) MCF-7 cell lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human bladder urothelial carcinoma tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human JK cells with MYDGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MYDGF antibody.
