A300-799A-T

antibody from Invitrogen Antibodies

Targeting: DHX33 DDX33, DKFZp762F2011, FLJ21972

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-799A-T

Provider

Invitrogen Antibodies

Product name

DHX33 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DHX33 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 (Cat. No. A300-799A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DHX33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX33 antibody A300-799A (lot A300-799A-2) used for IP at 6 µg per reaction. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody A300-800A For blotting immunoprecipitated DHX33, A300-799A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.