Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-2581 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2581, RRID:AB_2092147
- Product name
- Rabbit Polyclonal DHX33 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Loss of the deubiquitinase USP36 destabilizes the RNA helicase DHX33 and causes preimplantation lethality in mice.
The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
The E3 ubiquitin ligase tripartite motif 33 is essential for cytosolic RNA-induced NLRP3 inflammasome activation.
The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.
Identification of DHX33 as a mediator of rRNA synthesis and cell growth.
Fraile JM, Campos-Iglesias D, Rodríguez F, Astudillo A, Vilarrasa-Blasi R, Verdaguer-Dot N, Prado MA, Paulo JA, Gygi SP, Martín-Subero JI, Freije JMP, López-Otín C
The Journal of biological chemistry 2018 Feb 9;293(6):2183-2194
The Journal of biological chemistry 2018 Feb 9;293(6):2183-2194
The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Zhang Y, You J, Wang X, Weber J
Molecular and cellular biology 2015 Sep 1;35(17):2918-31
Molecular and cellular biology 2015 Sep 1;35(17):2918-31
The E3 ubiquitin ligase tripartite motif 33 is essential for cytosolic RNA-induced NLRP3 inflammasome activation.
Weng L, Mitoma H, Trichot C, Bao M, Liu Y, Zhang Z, Liu YJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3676-82
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3676-82
The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome.
Mitoma H, Hanabuchi S, Kim T, Bao M, Zhang Z, Sugimoto N, Liu YJ
Immunity 2013 Jul 25;39(1):123-35
Immunity 2013 Jul 25;39(1):123-35
Identification of DHX33 as a mediator of rRNA synthesis and cell growth.
Zhang Y, Forys JT, Miceli AP, Gwinn AS, Weber JD
Molecular and cellular biology 2011 Dec;31(23):4676-91
Molecular and cellular biology 2011 Dec;31(23):4676-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX33 Antibody [NB100-2581] - Detection of Human DHX33 on HeLa whole cell lystate using NB100-2581. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody NB100-2580.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX33 Antibody [NB100-2581] - Detection of Human and Mouse DHX33 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX33 antibody NB100-2581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DHX33 Antibody [NB100-2581] - Detection of human DHX33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2581 (lot 2) used for IP at 6 ug per reaction. DHX33 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-DHX33 antibody NB100-2580. For blotting immunoprecipitated DHX33, NB100-2581 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.