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- Antigen structure
- References [23]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 17219-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17219-1-AP, RRID:AB_2112476
- Product name
- GPD2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GPD2 antibody (Cat. #17219-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Targeting glycerophospholipid biosynthesis overcomes chemoresistance driven by SLFN11 loss in Ewing sarcoma.
The role of GPD2 and glycolysis-related genes in cholangiocarcinoma: insights into prognostic biomarkers and tumor-immune interactions.
D-lactate and glycerol as potential biomarkers of sorafenib activity in hepatocellular carcinoma.
MICU proteins facilitate Ca(2+)-dependent mitochondrial metabolon formation to regulate cellular energetics - independent of MCU.
AP1S3 affects lipid metabolism in breast cancer cells by regulating the PI3K/AKT/mTOR pathway.
FSCN1-mediated hepatic gluconeogenesis is indispensable for neonatal mice survival.
Species differences in glycerol-3-phosphate metabolism reveals trade-offs between metabolic adaptations and cell proliferation.
Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice.
Genistein and metformin regulate glycerol kinase and the enzymes of glycerol 3-phosphate shuttle in a differential manner in myocytes, hepatocytes and adipocytes.
Real-time monitoring of glucose metabolism and effects of metformin on HepG2 cells using (13)C in-cell NMR spectroscopy.
Histone Lactylation-Driven GPD2 Mediates M2 Macrophage Polarization to Promote Malignant Transformation of Cervical Cancer Progression.
Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.
Antioxidant Behavioural Phenotype in the Immp2l Gene Knock-Out Mouse.
Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis.
A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.
Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD(+) to alleviate mitochondrial disease.
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.
Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.
Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.
Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.
Chakraborty K, Burman R, Satheesh S, Kieffer M, Karuhatty C, Yuan ZF, Tan H, Lkhagva A, High AA, Wang X, Refaat A, Twarog NR, Zhang W, Wang Y, Fan Y, Li Q, Madan Babu M, Shelat AA, Stewart E, Dyer MA, Bagga P
Cell death & disease 2026 Jan 31;17(1):190
Cell death & disease 2026 Jan 31;17(1):190
The role of GPD2 and glycolysis-related genes in cholangiocarcinoma: insights into prognostic biomarkers and tumor-immune interactions.
Zhao Y, Zhu W, Ren B, Qin W, Zhou W
Cancer cell international 2025 Oct 9;25(1):345
Cancer cell international 2025 Oct 9;25(1):345
D-lactate and glycerol as potential biomarkers of sorafenib activity in hepatocellular carcinoma.
Pedretti S, Palermo F, Braghin M, Imperato G, Tomaiuolo P, Celikag M, Boccazzi M, Vallelonga V, Da Dalt L, Norata GD, Marisi G, Rapposelli IG, Casadei-Gardini A, Ghisletti S, Crestani M, De Fabiani E, Mitro N
Signal transduction and targeted therapy 2025 Jun 27;10(1):200
Signal transduction and targeted therapy 2025 Jun 27;10(1):200
MICU proteins facilitate Ca(2+)-dependent mitochondrial metabolon formation to regulate cellular energetics - independent of MCU.
Cohen HM, Gottschalk B, Choya-Foces C, Chathoff A, Wilkinson A, Garbincius JF, Johnson A, Stevens TL, Howe JE, Megill E, Ngo J, Tomar D, Snyder NW, Graier WF, Elrod JW
Research square 2025 Jun 26;
Research square 2025 Jun 26;
AP1S3 affects lipid metabolism in breast cancer cells by regulating the PI3K/AKT/mTOR pathway.
Wu Z, Wang S, Yang Q, Gong Y, Hu J
Biochemical and biophysical research communications 2025 Aug 30;776:152200
Biochemical and biophysical research communications 2025 Aug 30;776:152200
FSCN1-mediated hepatic gluconeogenesis is indispensable for neonatal mice survival.
Liu X, Hu Y, Wu L, Zhang Y, Sang L, Gao Y, He L, Xiong W, Yang S, Sun J
Acta biochimica et biophysica Sinica 2025 Aug 29;58(3):584-594
Acta biochimica et biophysica Sinica 2025 Aug 29;58(3):584-594
Species differences in glycerol-3-phosphate metabolism reveals trade-offs between metabolic adaptations and cell proliferation.
Gaertner K, Terzioglu M, Michell C, Tapanainen R, Pohjoismäki J, Dufour E, Saari S
Biochimica et biophysica acta. Bioenergetics 2025 Apr 1;1866(2):149530
Biochimica et biophysica acta. Bioenergetics 2025 Apr 1;1866(2):149530
Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice.
Park SY, Jung SR, Kim JY, Kim YW, Sung HK, Park SY, Doh KO, Koh JH
American journal of physiology. Cell physiology 2024 Sep 1;327(3):C619-C633
American journal of physiology. Cell physiology 2024 Sep 1;327(3):C619-C633
Genistein and metformin regulate glycerol kinase and the enzymes of glycerol 3-phosphate shuttle in a differential manner in myocytes, hepatocytes and adipocytes.
Syngkli S, Singh SK, Rani RM, Das B
International journal of biological macromolecules 2024 Jun;270(Pt 1):132296
International journal of biological macromolecules 2024 Jun;270(Pt 1):132296
Real-time monitoring of glucose metabolism and effects of metformin on HepG2 cells using (13)C in-cell NMR spectroscopy.
Teng M, Li Z, Gu Y, Fan Y, Wang D, Liu M, Li Y, Wei G, Huang Y
Biochemical and biophysical research communications 2024 Jan 29;694:149383
Biochemical and biophysical research communications 2024 Jan 29;694:149383
Histone Lactylation-Driven GPD2 Mediates M2 Macrophage Polarization to Promote Malignant Transformation of Cervical Cancer Progression.
Huang C, Xue L, Lin X, Shen Y, Wang X
DNA and cell biology 2024 Dec;43(12):605-618
DNA and cell biology 2024 Dec;43(12):605-618
Mitochondrial Glycerol-3-Phosphate Dehydrogenase Restricts HBV Replication via the TRIM28-Mediated Degradation of HBx.
Liu C, Zhao K, Chen Y, Yao Y, Tang J, Wang J, Xu C, Yang Q, Zheng Y, Yuan Y, Sun H, Zhang Y, Zhou Y, Chen J, Wang Y, Wu C, Pei R, Chen X
Journal of virology 2023 May 31;97(5):e0058023
Journal of virology 2023 May 31;97(5):e0058023
Antioxidant Behavioural Phenotype in the Immp2l Gene Knock-Out Mouse.
Lawther AJ, Zieba J, Fang Z, Furlong TM, Conn I, Govindaraju H, Choong LLY, Turner N, Siddiqui KS, Bridge W, Merlin S, Hyams TC, Killingsworth M, Eapen V, Clarke RA, Walker AK
Genes 2023 Aug 28;14(9)
Genes 2023 Aug 28;14(9)
Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis.
Yao CH, Park JS, Kurmi K, Hu SH, Notarangelo G, Crowley J, Jacobson H, Hui S, Sharpe AH, Haigis MC
Molecular cell 2023 Apr 20;83(8):1340-1349.e7
Molecular cell 2023 Apr 20;83(8):1340-1349.e7
A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.
Wu S, Mao C, Kondiparthi L, Poyurovsky MV, Olszewski K, Gan B
Proceedings of the National Academy of Sciences of the United States of America 2022 Jun 28;119(26):e2121987119
Proceedings of the National Academy of Sciences of the United States of America 2022 Jun 28;119(26):e2121987119
Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD(+) to alleviate mitochondrial disease.
Liu S, Fu S, Wang G, Cao Y, Li L, Li X, Yang J, Li N, Shan Y, Cao Y, Ma Y, Dong M, Liu Q, Jiang H
Cell metabolism 2021 Oct 5;33(10):1974-1987.e9
Cell metabolism 2021 Oct 5;33(10):1974-1987.e9
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10
eLife 2021 May 26;10
Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.
Langston PK, Nambu A, Jung J, Shibata M, Aksoylar HI, Lei J, Xu P, Doan MT, Jiang H, MacArthur MR, Gao X, Kong Y, Chouchani ET, Locasale JW, Snyder NW, Horng T
Nature immunology 2019 Sep;20(9):1186-1195
Nature immunology 2019 Sep;20(9):1186-1195
Low metformin causes a more oxidized mitochondrial NADH/NAD redox state in hepatocytes and inhibits gluconeogenesis by a redox-independent mechanism.
Alshawi A, Agius L
The Journal of biological chemistry 2019 Feb 22;294(8):2839-2853
The Journal of biological chemistry 2019 Feb 22;294(8):2839-2853
Obesity and steatosis promotes mitochondrial remodeling that enhances respiratory capacity in the liver of ob/ob mice.
Garcia J, Decker CW, Sanchez SJ, Ouk JM, Siu KM, Han D
FEBS letters 2018 Mar;592(6):916-927
FEBS letters 2018 Mar;592(6):916-927
Mitochondrial remodeling in the liver following chronic alcohol feeding to rats.
Han D, Johnson HS, Rao MP, Martin G, Sancheti H, Silkwood KH, Decker CW, Nguyen KT, Casian JG, Cadenas E, Kaplowitz N
Free radical biology & medicine 2017 Jan;102:100-110
Free radical biology & medicine 2017 Jan;102:100-110
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, Eagan J, Desai NK, Newton SA, Towne MC, Marinakis NS, Esteves KM, De Ferranti S, Bennett MJ, McIntyre A, Beggs AH, Berry GT, Agrawal PB
European journal of human genetics : EJHG 2014 Oct;22(10):1229-32
European journal of human genetics : EJHG 2014 Oct;22(10):1229-32
A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase.
Orr AL, Quinlan CL, Perevoshchikova IV, Brand MD
The Journal of biological chemistry 2012 Dec 14;287(51):42921-35
The Journal of biological chemistry 2012 Dec 14;287(51):42921-35
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17219-1-AP(GPD2 antibody) at dilution of 1:800
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 17219-1-AP(GPD2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GPD2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GPD2. This antibody recognizes human,mouse,rat antigen. The GPD2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
