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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-776A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIS12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody A300-776A (lot A300-776A-2) used for IP at 3 µg per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody A300-775A. For blotting immunoprecipitated MIS12, A300-776A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody A300-776A (lot A300-776A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (Cat. No. A300-776A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
