PA5-118064

antibody from Invitrogen Antibodies

Targeting: MIS12 hMIS12, KNTC2AP, MGC2488, MTW1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118064

Provider

Invitrogen Antibodies

Product name

MIS12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MIS12 Polyclonal Antibody (Product # PA5-118064) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MIS12 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 µL with MIS12 Polyclonal Antibody (Product # PA5-118064) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MIS12 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MIS12 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 µL with MIS12 Polyclonal Antibody (Product # PA5-118064) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MIS12 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.