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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-2359 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2359, RRID:AB_10001410
- Product name
- Rabbit Polyclonal MIS12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment.
CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.
Liu XS, Song B, Tang J, Liu W, Kuang S, Liu X
Molecular and cellular biology 2012 Oct;32(19):4053-67
Molecular and cellular biology 2012 Oct;32(19):4053-67
CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.
Maehara K, Takahashi K, Saitoh S
Molecular and cellular biology 2010 May;30(9):2090-104
Molecular and cellular biology 2010 May;30(9):2090-104
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MIS12 Antibody [NB100-2359] - Detection of Human MIS12 on HeLa whole cell lysate using NB100-235. MIS12 was also immunoprecipitated by rabbit another anti-MIS12 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody NB100-2358. For blotting immunoprecipitated MIS12, NB100-2359 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MIS12 Antibody [NB100-2359] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-MIS12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). Detection: DAB
