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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-14967 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GCS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references p32 regulates ER stress and lipid homeostasis by down-regulating GCS1 expression.
Liu Y, Leslie PL, Jin A, Itahana K, Graves LM, Zhang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3892-3902
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3892-3902
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band recognized by the antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A beads. The eluted proteins were analyzed through 12.5% SDS PAGE. Arrow indicates GCS1 band. Image from verified customer review.
