PA5-89695

antibody from Invitrogen Antibodies

Targeting: GABRG1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89695

Provider

Invitrogen Antibodies

Product name

GABRG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HSCPLEFSSY GYPKNEIEYK WKKPSVEVAD PKYWRLYQFA FVGLRNSTEI THTISGDYVI M; Positive Samples: U-87MG, Mouse brain; Cellular Location: Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.88 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GABRG1 in extracts of various cell lines. Samples were incubated with GABRG1 Polyclonal antibody (Product # PA5-89695) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GABRG1 in C6 cells. Samples were incubated with GABRG1 Polyclonal antibody (Product # PA5-89695) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.