AF8555

antibody from Novus Biologicals

Targeting: A4GNT alpha4GnT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8555

Provider

Novus Biologicals

Product name

Rabbit Polyclonal A4GNT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human alpha-1,4-N-Acetylglucosaminyltransferase in direct ELISAs and Western blots.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

12 months from date of receipt, -20 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after opening. 6 months, -20 degreesC under sterile conditions after opening.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO-III human gastric carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.