Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB03522 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- ABCG1 antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
Submitted references ABCA12 regulates ABCA1-dependent cholesterol efflux from macrophages and the development of atherosclerosis.
Fu Y, Mukhamedova N, Ip S, D'Souza W, Henley KJ, DiTommaso T, Kesani R, Ditiatkovski M, Jones L, Lane RM, Jennings G, Smyth IM, Kile BT, Sviridov D
Cell metabolism 2013 Aug 6;18(2):225-38
Cell metabolism 2013 Aug 6;18(2):225-38
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ABCG1 antibody (N-term) in mouse spleen tissue lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.
- Sample type
- mouse spleen tissue lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ABCG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human lung carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ABCG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- HepG2 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol