PA5-56757

antibody from Invitrogen Antibodies

Targeting: ABCG1 ABC8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56757

Provider

Invitrogen Antibodies

Product name

ABCG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AEMTEPKSVC VSVDEVVSSN MEATETDLLN GHLKKVDNNL TEAQRFSSLP RRAAVNIEFR DLSYSVPEG

Concentration

0.1 mg/mL

References

Submitted references

Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis.

Maras JS, Das S, Bhat A, Kumar Vyas A, Yadav G, Chaudhary S, Sukriti S, Gupta AC, Bihari C, Mahiwall R, Sarin SK
Hepatology communications 2019 Dec;3(12):1598-1625

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ABCG1 Polyclonal Antibody (Product # PA5-56757) and a ~60 kDa band corresponding to ABCG1 was observed across cell lines tested . Membrane enriched extracts (50 µg lysate) of THP-1 (Lane 1), THP-1 treated with 50 ng/mL TPA for 24h (Lane 2), THP-1 treated with TPA (50 ng/mL) along with IL-33 (10 ng/mL) for 24h (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Expression of ABCG1 Polyclonal antibody (Product # PA5-56757) was observed in THP-1 cells upon treatment with 50 ng/mL TPA for 24h (Lane 2) and with 50 ng/mL TPA for 24h along with 10 ng/mL IL 33 for 24 h (Lane 3).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ABCG1 in human cell line HeLa shows positivity in the Golgi apparatus, vesicles & nucleus but excluded from the nucleoli. Samples were probed using an ABCG1 Polyclonal Antibody (Product # PA5-56757).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Quantitative proteomic analysis identifies significantly down-regulated lipid transporter proteins in SAH. (A) Partial list of proteins differentially regulated (>1.5-fold; P < 0.05) between SAH, AC, HC, and its GO analysis, which documents enrichment of lipid transporters in SAH. (B) KEGG enrichment analysis of the up-regulated (n = 32) and down-regulated (n = 176) proteins in patients with SAH represents significant ( P < 0.05) enrichment. (C) Plasma level of PON1 was down-regulated in patients with SAH (median, 23.2; range, 2.3-139.4) compared to those with AC (median, 44.7; range, 25.8-131) and HC (median, 139.5; range, 46.6-196.0) ( P < 0.05). (D) Plasma level of apolipoproteins (APOB, APOE, APOA1, APOA2, APOC1, APOC3) in SAH compared to AC and HC ( P < 0.05 is significant). (E) IHC showing expression of PON1, LDL, CD36, SRA1, ABCA1, and ABCG1 in SAH (n = 5) and AC (n = 5). For all IHC analyses, relative quantization of positively stained cells are expressed as mean number of positive cells/10 high-power field (40x) and * P < 0.05. Abbreviations: COA, coenzyme A; HBG1, hemoglobin subunit gamma 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LBP, lipopolysaccharide binding protein; LPS, lipopolysaccharide; TLR4, toll-like receptor 4;