Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2504 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with rhCA1, -2, -3, -6, -8, -10, -13, recombinant mouse CA4, -9, and -12 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9WVT6
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CO2 chemosensing in rat oesophagus.
Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
Gut 2008 Dec;57(12):1654-64
Gut 2008 Dec;57(12):1654-64
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Carbonic Anhydrase XIV/CA14 by Western Blot. Western blot shows lysates of human, mouse, and rat brain (cerebellum) tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Carbonic Anhydrase XIV/CA14 by Simple WesternTM. Simple Western lane view shows lysates of mouse and rat brain (cerebellum) tissue and Hepa 1-6 mouse hepatoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/ CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.