Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-29069 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase XIV Recombinant Rabbit Monoclonal Antibody (515)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human Carbonic Anhydrase XIV/CA14.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 515
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human Carbonic Anhydrase XIV in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Carbonic Anhydrase XIV Recombinant Rabbit Monoclonal Antibody (515) (Product # MA5-29069, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Carbonic Anhydrase XIV in human liver with Carbonic Anhydrase XIV Recombinant Rabbit Monoclonal Antibody (515) (Product # MA5-29069, 1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte.