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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-31286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMELX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, NIH-3T3.
- Concentration
- 1.22 mg/mL
Submitted references Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells.
Robertson SYT, Wen X, Yin K, Chen J, Smith CE, Paine ML
Frontiers in physiology 2017;8:336
Frontiers in physiology 2017;8:336
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AMELX Polyclonal Antibody (Product # PA5-31286). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 12% SDS PAGE. AMELX Polyclonal Antibody (Product # PA5-31286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AMELX Polyclonal Antibody (Product # PA5-31286). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 12% SDS PAGE. AMELX Polyclonal Antibody (Product # PA5-31286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Western blot analyses of PMCA1-4, and NCKX3 in secretory-stage and maturation-stage rat enamel organs. (A) Western blot analysis for PMCA1 (Ai) , PMCA2 (Aii) , PMCA3 (Aiii) and PMCA4 (Aiv) . Samples are secretory-stage enamel organ cells (S), maturation-stage enamel organ cells (M), brain tissue (B) and heart tissue (H). Brain and heart samples are shown for comparison, as all PMCAs are highly expressed in brain and at lower levels in the heart (Brini and Carafoli, 2011 ; Brini et al., 2017 ). Molecular weight markers are indicated at left. The expected molecular weights for PMCA1, PMCA2, PMCA3, and PMCA4 are ~130, 133, 123, and 129 kDa, respectively. The bands are seen for PMCA1, PMCA3, and PMCA4 (boxed and arrow). No expression of PMCA2 is evident. GAPDH is used here as a loading control. (B) Western blot analysis of NCKX3. The expected molecular weight for NCKX3 is ~60 kDa. NCKX3 is expressed in all 4 tissue samples tested, with similar expression noted in both secretory-stage and maturation-stage enamel organ cells, and brain tissue. Relatively higher levels of NCKX3 expression can be appreciated in heart tissue. Amelx and Actc are used as controls as expression is highest in secretory-stage ameloblasts (Lacruz et al., 2012a , b ) and heart tissue (Hamada et al., 1982 ) respectively. GAPDH is used here as a loading control.
