PA5-31286

antibody from Invitrogen Antibodies

Targeting: AMELX AIH1, AMG

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31286

Provider

Invitrogen Antibodies

Product name

AMELX Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, NIH-3T3. Predicted reactivity: Mouse (81%), Rat (81%), Pig (82%), Rhesus Monkey (98%), Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.22 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells.

Robertson SYT, Wen X, Yin K, Chen J, Smith CE, Paine ML
Frontiers in physiology 2017;8:336

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Western blot analyses of PMCA1-4, and NCKX3 in secretory-stage and maturation-stage rat enamel organs. (A) Western blot analysis for PMCA1 (Ai) , PMCA2 (Aii) , PMCA3 (Aiii) and PMCA4 (Aiv) . Samples are secretory-stage enamel organ cells (S), maturation-stage enamel organ cells (M), brain tissue (B) and heart tissue (H). Brain and heart samples are shown for comparison, as all PMCAs are highly expressed in brain and at lower levels in the heart (Brini and Carafoli, 2011 ; Brini et al., 2017 ). Molecular weight markers are indicated at left. The expected molecular weights for PMCA1, PMCA2, PMCA3, and PMCA4 are ~130, 133, 123, and 129 kDa, respectively. The bands are seen for PMCA1, PMCA3, and PMCA4 (boxed and arrow). No expression of PMCA2 is evident. GAPDH is used here as a loading control. (B) Western blot analysis of NCKX3. The expected molecular weight for NCKX3 is ~60 kDa. NCKX3 is expressed in all 4 tissue samples tested, with similar expression noted in both secretory-stage and maturation-stage enamel organ cells, and brain tissue. Relatively higher levels of NCKX3 expression can be appreciated in heart tissue. Amelx and Actc are used as controls as expression is highest in secretory-stage ameloblasts (Lacruz et al., 2012a , b ) and heart tissue (Hamada et al., 1982 ) respectively. GAPDH is used here as a loading control.