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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-28282 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MSL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse muscle. Predicted reactivity: Mouse (95%), Rat (94%), Xenopus laevis (80%), Pig (96%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MSL3 Polyclonal Antibody detects MSL3L1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSL3L1 protein stained by MSL3 Polyclonal Antibody (Product # PA5-28282) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MSL3 Polyclonal Antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 µg of MSL3 Polyclonal Antibody (Product # PA5-28282) 7.5% SDS-PAGE The immunoprecipitated MSL3L1 protein was detected by MSL3 Polyclonal Antibody (Product # PA5-28282) diluted at 1:1,000.
