Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-57446 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COMMD8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: SRKDEIKQAL SREIVAISSA QLQDFDWQVK LALSSDKIAA LRMPLLSLHL DVKENGEVKP YSIEMSREEL QNLIQSLEAA NKVVLQLK Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 84%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COMMD8 in control (vector only transfected HEK293T lysate) and COMMD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a COMMD8 Polyclonal Antibody (Product # PA5-57446).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of COMMD8 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a COMMD8 Polyclonal Antibody (Product # PA5-57446).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of COMMD8 in human cell line U-251 MG using COMMD8 Polyclonal Antibody (Product # PA5-57446). Staining shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COMMD8 in human cerebral cortex using COMMD8 Polyclonal Antibody (Product # PA5-57446) shows moderate cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COMMD8 in human pancreas using COMMD8 Polyclonal Antibody (Product # PA5-57446) shows strong cytoplasmic positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COMMD8 in human rectum using COMMD8 Polyclonal Antibody (Product # PA5-57446) shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COMMD8 in human skin using COMMD8 Polyclonal Antibody (Product # PA5-57446) shows strong cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COMMD8 in human cerebral cortex using COMMD8 Polyclonal Antibody (Product # PA5-57446) shows moderate cytoplasmic positivity in neurons.
