PA5-96738

antibody from Invitrogen Antibodies

Targeting: CENPK CENP-K, FKSG14, SOLT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96738

Provider

Invitrogen Antibodies

Product name

CENPK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MNQEDLDPDS TTDVGDVTNT EEELIRECEE MWKDMEECQN KLSLIGTETL TDSNAQLSLL IMQVKCLTAE LSQWQKKTPE TIPLTEDVLI TLGKEEFQKL RQDLEMVLST KESKNEKLKE DLEREQRWLD EQQQIMESLN VLHSELKNKV ETFSESRIFN ELKTKMLNIK EYKEKLLSTL GEFLEDHFPL PDRSVKKKKK NIQESSVNLI TLHEMLEILI NRLFDVPHDP YVKISDSFWP PYVELLLRNG IALRHPEDPT RIRLEAFHQ; Positive Samples: HeLa, SKOV3, 293T; Cellular Location: Chromosome, Nucleus, centromere, kinetochore

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.62 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CENPK in extracts of various cell lines using CENPK Polyclonal Antibody (Product # PA5-96738) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CENPK was performed in U2OS cells using CENPK Polyclonal Antibody (Product # PA5-96738).