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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-97578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CENPK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated CENPK from Hela whole cell lysate using a CENPK polyclonal antibody (Product # PA5-97578). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated CENPK from Hela whole cell lysate using a CENPK polyclonal antibody (Product # PA5-97578). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CENPK using a CENPK Polyclonal antibody (Product # PA5-97578) at a concentration of 3.98 µg/mL. Lane 1: PC-3 whole cell lysate. Lane 2: 293T whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 32 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CENPK in paraffin embedded human skin tissue using a CENPK polyclonal antibody (Product # PA5-97578) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CENPK in paraffin embedded human liver tissue using a CENPK polyclonal antibody (Product # PA5-97578) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CENPK in paraffin embedded human skin tissue using a CENPK polyclonal antibody (Product # PA5-97578) at a dilution of 1:100.
