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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUCLA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SUCLA2 Polyclonal Antibody (Product # PA5-22051). Sample (30 µg of whole cell lysate). Lane A: Hep G2. Lane B: Molt-4. 10% SDS PAGE. SUCLA2 Polyclonal Antibody (Product # PA5-22051) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SUCLA2 Polyclonal Antibody (Product # PA5-22051). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. SUCLA2 Polyclonal Antibody (Product # PA5-22051) diluted at 1:20,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SUCLA2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: SUCLA2 Polyclonal Antibody (Product # PA5-22051) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SUCLA2 (Product # PA5-22051) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SUCLA2 Polyclonal Antibody detects SUCLA2 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SUCLA2 Polyclonal Antibody (Product # PA5-22051) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
