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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- MAB2209-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Corin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Corin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Q5- Isotype
- IgG
- Antibody clone number
- 231443
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells.
Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H
Scientific reports 2016 Sep 9;6:32532
Scientific reports 2016 Sep 9;6:32532
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Corin ELISA Standard Curve. Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Corin in HK2 Human Cell Line. Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
