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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- AF2209 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Corin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Corin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Q5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation.
Kaushal GS, Rognoni E, Lichtenberger BM, Driskell RR, Kretzschmar K, Hoste E, Watt FM
The Journal of investigative dermatology 2015 Dec;135(12):2926-2934
The Journal of investigative dermatology 2015 Dec;135(12):2926-2934
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Corin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # af2209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Corin at approximately 85 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Corin ELISA Standard Curve. Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Corin in Human Heart. Corin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Corin in Human Liver. Corin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
