Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4407 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TFF3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q07654
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia.
Ge H, Gardner J, Wu X, Rulifson I, Wang J, Xiong Y, Ye J, Belouski E, Cao P, Tang J, Lee KJ, Coberly S, Wu X, Gupte J, Miao L, Yang L, Nguyen N, Shan B, Yeh WC, VĂ©niant MM, Li Y, Baribault H
PloS one 2015;10(6):e0126924
PloS one 2015;10(6):e0126924
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human TFF3 by Western Blot. Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TFF3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.