Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- HPA027850 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA027850, RRID:AB_10600868
- Product name
- Anti-DMRT1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DMRT1, Gene description: doublesex and mab-3 related transcription factor 1, Alternative Gene Names: CT154, DMT1, Validated applications: IHC, WB, Uniprot ID: Q9Y5R6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals
The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia
Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia
Richardson N, Gillot I, Gregoire E, Youssef S, de Rooij D, de Bruin A, De Cian M, Chaboissier M
eLife 2020;9
eLife 2020;9
The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Djureinovic D, Fagerberg L, Hallström B, Danielsson A, Lindskog C, Uhlén M, Pontén F
MHR: Basic science of reproductive medicine 2014;20(6):476-488
MHR: Basic science of reproductive medicine 2014;20(6):476-488
Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia
von Kopylow K, Staege H, Spiess A, Schulze W, Will H, Primig M, Kirchhoff C
REPRODUCTION 2012;143(1):45-57
REPRODUCTION 2012;143(1):45-57
Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia
Kopylow K, Staege H, Schulze W, Will H, Kirchhoff C
Histochemistry and Cell Biology 2012;138(5):759-772
Histochemistry and Cell Biology 2012;138(5):759-772
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DMRT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411862).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and fallopian tube tissues using HPA027850 antibody. Corresponding DMRT1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol