Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- RP1083 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-DMRT1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for DMRT1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. DMRT1 information: Molecular Weight: 39473 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Testis-specific. Expressed in prostate cancer (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson's Disease Model Induced by 6-OHDA.
Nuclear import of human sexual regulator DMRT1 is mediated by importin-beta.
Tai S, Zheng Q, Zhai S, Cai T, Xu L, Yang L, Jiao L, Zhang C
Frontiers in neuroscience 2020;14:612
Frontiers in neuroscience 2020;14:612
Nuclear import of human sexual regulator DMRT1 is mediated by importin-beta.
Ying M, Chen B, Tian Y, Hou Y, Li Q, Shang X, Sun J, Cheng H, Zhou R
Biochimica et biophysica acta 2007 Jun;1773(6):804-13
Biochimica et biophysica acta 2007 Jun;1773(6):804-13
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).DMRT1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image