H00001761-M01

antibody from Abnova Corporation

Targeting: DMRT1 CT154, DMT1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001761-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001761-M01, RRID:AB_437029

Product name

DMRT1 monoclonal antibody (M01), clone 1G11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant DMRT1.

Antigen sequence

MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKAS
GALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKS
PRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKC
NLIAERQRVMAAQVALRRQQAQEEELGISHPIPLP
SAAELLVKRENNGSNPCLMTECSGTSQPPPASVPT
TAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYY
SSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVG
NPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMEN
RHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPS
YPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKA
VLECEPASEPSSFTVTPVIEEDE


Isotype

IgG

Antibody clone number

1G11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DMRT1 monoclonal antibody (M01), clone 1G11 Western Blot analysis of DMRT1 expression in HL-60 ( Cat # L014V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DMRT1 expression in transfected 293T cell line by DMRT1 monoclonal antibody (M01), clone 1G11.Lane 1: DMRT1 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DMRT1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol